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Biochemical blood test: total protein, albumin, total and indirect bilirubin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), alkaline phosphatase, creatinine, urea, uric acid, calcium, phosphorus, iron, ferritin, glucose, and total cholesterol. This analysis is necessary to evaluate the function of the liver and kidneys, detect abnormalities in phosphorus-calcium (relevant for chronic kidney disease CKD) and iron metabolism. 5.