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The spot is slightly yellow as the product is yellow. In the Co (combined) lane, there is a NS hat on the BA. Only one spot of nitrostyrene in the NS lane, product seems to be pure, the reaction is a success. During the course of the reaction, monitoring on TLC, you will see that the hat (nitrostyrene product) will become brighter and the head (3,4,5- TMBA) will fade. 16) From the freezer collect the second crop of crystals.